SOP.PRO.05.09 QUIZ (Processor)

True or False, if there any any clots in the PRBC unit the plasma cannot be used to manufacture cryoprecipitate.

True
False

To manufacture cryo from a unit of fresh frozen plasma, what is the minimum FFP unit weight required?

160 grams
200 grams
240 grams

Which of the following labelling configurations is correct when manufacturing cryo?

Platelet Bag – Write the donor number and CP Plasma Bag – Write CS
Platelet Bag – Write the donor number and CS Plasma Bag – Write CP

With regards to freezing FFP to manufacture cryo which of the following statements is FALSE?

The FFP must be frozen within 25 hours of donation
A 200 ml unit of FFP takes approximately 45 minutes to freeze
If the FFP is removed from storage to defrost for cryo production and was at room temperature for 5 hours before it became apparent that cryo could not be manufactured that day, it could be re-frozen again as FFP to use another day.

When completing the storage logs and generating a product label after completing FFP production to be used to manufacture cryo which if the following statements is FALSE?

CRYO DO NOT SELL should be entered into Orderwise in supplier advice and 'CRYO' should be entered in the lab comments section of the FFP storage log
The CRYO label generated in Orderwise should be secured on the FFP unit once frozen
The FFP unit should be placed in the freezer in a bubble wrap pouch and zip lock bag until it is ready to be processed into cryo

When defrosting FFP for cryo production which two of the following statements is correct?

When removing 4 units for cryo manufacture, the time they are removed from the freezer should be staggered to prevent some of the units over thawing
The FFP units should be defrosted in a warm water bath and agitated regularly to reduce defrost time
A batch of at least 8 FFP units should be defrosted each time for manufacture as manufacturing fewer units is less efficient
Defrosting the FFP units takes between 4 – 8 hours to reach the desired slushy consistency
FFP should be defrosted at rom temperature by laying the units out flat on the top of the freezer

When setting up the lab to manufacture cryo, the blast freezer should be switched on and programmed ready, the bench should be clean and the scales calibrated and the log completed. The centrifuge should be programmed and may need to be run through a few cycles to reduce the temperature. Which of the following is the correct temperature, speed and duration?

4 degrees C, 3840 RPM for 5 minutes
4 degrees C, 1240 RPM for 15 minutes
4 degrees C, 3840 RPM, for 15 minutes
6 degrees C, 4200 RPM for 10 minutes
6 degrees C, 3840 RPM for 5 minutes

When centrifuging the FFP for cryo manufacture which of the following instructions is TRUE?

The ports should be at the open end of the zip lock bag and the top when placed in the centrifuge bucket
The FFP should contain large lumps of frozen plasma still when placed in the centrifuge
The FFP label should face the outside of the centrifuge

Which of the following is correct? If the volume of FFP in a bag weighs 285 grams …….

The Cryosupernatant portion of that will be 95 grams
The cryoprecipitate portion of that will be 190 grams
The Cryosupernatant portion of that will be 285 grams
The cryoprecipitate portion of that will be 95 grams

In relation to the transfer of the now spun FFP and the extraction of the Cryosupernatant which of the following is FALSE?

Great care should be taken when removing the spun FFP from the centrifuge to keep the unit upright and reduce movement
When the plasma press is engaged the cryoprecipitate will flow through the ice barrier into the platelet bag
A maximum of two FFPs units should be removed from the centrifuge to be processed the rest should stay in there with the lid on to keep them cool
The plasma bag will need to be straightened out after being spun in the centrifuge. This is done by holding the outlet port and pulling the bag down by the hanging loop at the bottom of the bag

Once the Cryosupernatant has been transferred to the platelet bag, line C should be clamped to stop further plasma flow. The sides of the plasma bag should be rubbed to ensure all the cryoprecipitate is mixed and the ice dispersed.
Using the blood bag configuration chart, which lines now need to be heat sealed next to the junction and then further heat sealed (after stripping) to create 3 aliquots of cryo-s and 3 of cryo-p?

A and C
B and D
C and E

Line F has been discarded and each cryo unit weighed and the volumes calculated and the cryo process log updated. Identify all 5 of the following statements about the rest of the process that are correct?

One aliquot from each cryo unit is detached and taped to the top of the corresponding unit e.g. cryo-s aliquot to cryo-s unit and then the units and placed in the freezer
Orderwise is updated using the completed cryo process log and the labels can then be printed
The expiry dates of the product should be checked to ensure hey are correct. Both cryo-s and cryo-p have a 2 year expiry date
The units and the aliquot can be labelled
The cryo units can be placed into an individual bubble wrap bag and placed into the plasma freezer
The labelled separate cryo aliquots should be secured together with an elastic band and labelled with the date and stored with the cryo units in the freezer
The centrifuge should be run on a cycle at the 22 degree C Programme and the lab and equipment cleaned and put away

SOP.PRO.05.09 QUIZ (Processor)

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